Journal: Molecular Cancer

Article Title: Gene expression time-series analysis of Camptothecin effects in U87-MG and DBTRG-05 glioblastoma cell lines

doi: 10.1186/1476-4598-7-66

Figure Lengend Snippet: Semi-quantitative RT-PCR post-validation of microarray results . A . Gene expression changes for seven genes (CDKN1A, GADD45, TXNIP, MYC, GEM, AREG, EREG) were measured at selected time points after CPT treatment by semi-quantitative RT-PCR and normalized to β-actin expression of time- and dose- matched controls. The RT-PCR reactions were analyzed with the DNA1000 kit of the Agilent 2100 Bioanalyzer to give a gel-like image. B . At each selected time point after CPT treatment, expression fold change values obtained with semi-quantitative RT-PCR and microarrays were compared.

Article Snippet: Slides were scanned in both Cy-3 and Cy-5 channels with Agilent dual laser microarray scanner (G2565AA).

Techniques: Quantitative RT-PCR, Microarray, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: ACS Omega

Article Title: Increased Retention of Cardiac Cells to a Glass Substrate through Streptavidin–Biotin Affinity

doi: 10.1021/acsomega.1c02003

Figure Lengend Snippet: Functionalization of a glass substrate to increase cell retention. (A) Epoxy coating of a glass substrate followed by streptavidin conjugation and binding of biotinylated cells. (B) Fluorescent imagining of SA-Cy3 coating on epoxy slides and negative control/unmodified slides. Streptavidin density determined using the Cy3 calibration slide (scale bar = 1 mm).

Article Snippet: Using a Cy3 Full Moon Biosystems scanner calibration slide, the fluorescent signal obtained from an Affymetrix 428 Array scanner could be directly related to the fluorophore density on the glass surface.

Techniques: Conjugation Assay, Binding Assay, Negative Control

Journal: BMC Genetics

Article Title: Genetic and transcriptomic analysis of transcription factor genes in the model halophilic Archaeon: coordinate action of TbpD and TfbA

doi: 10.1186/1471-2156-8-61

Figure Lengend Snippet: Microarray images. Identical regions of one of the Δ tbp D and Δ tfb A microarrays are shown (panels A and B, respectively). C. Grid representing the probes that have a fold change value greater than or equal to 1.5 in all four microarrays performed (both biological and technical replicates for Δ tbp D and Δ tfb A).

Article Snippet: Slides were scanned for Cy -3 and Cy -5 signals with an Agilent DNA-microarray scanner.

Techniques: Microarray

Journal: BMC Genetics

Article Title: Genetic and transcriptomic analysis of transcription factor genes in the model halophilic Archaeon: coordinate action of TbpD and TfbA

doi: 10.1186/1471-2156-8-61

Figure Lengend Snippet: Scatter plot of microarray data from transcription factor knockouts. (A) A plot of the fold-change of each gene in the Δ tbp D microarrays. The most up and down regulated genes and some known heat shock response genes are highlighted. (B) A plot of the fold-change of each gene in the Δ tfb A microarrays. The most up and down regulated genes and some known heat shock response genes are highlighted.

Article Snippet: Slides were scanned for Cy -3 and Cy -5 signals with an Agilent DNA-microarray scanner.

Techniques: Microarray